RM0.00
Virus RNA RT-qPCR Detection Kit for RUO
Price RM0.00
Product SKU QD302-02
Brand Vazyme Biotech
Availability In Stock
Description
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This product provides triplex-detections in a single tube. Specific primers and probes were designed for the detection of conserved region of Virus O gene and N gene, respectively. Internal control (RNAse P gene) provides a nucleic acid extraction procedural control and a secondary negative control. Positive control provides a nucleic acid extraction and a reverse transcription control to validate the entire procedure and reagent

This product is a multiplex fluorescent probe-based Taqman RT-qPCR assay system. The Taqman fluorescent probe is a specific oligonucleotide based on a reporter-quencher mechanism. For each probe, the 5’end is labeled with a fluorophore, while the 3’end was labeled with a quencher. When the probe is intact, the fluorescence emitted by the fluorophore is absorbed by the quencher, and no fluorescent signal is detected. However, during amplification of the template, the probe will be degraded due to the 5'-3’ exonuclease activity of Taq DNA polymerase, and the fluorescent reporter and the quencher are cleaved and separated, then a fluorescent signal can be detected. The generation of each molecular amplicon is accompanied by the generation of a fluorescent signal. Real-time monitoring of the entire PCR process can be assessed by monitoring the accumulation of fluorescent signals.

INSTRUMENTS:

Real-time PCR instrument with FAM, TEXAS RED/ROX and HEX/VIC channels, such as ABI7500, ABI Q3, ABI Q6, Bio-Rad CFX96.

SAMPLING & HANDLING:

1. The collected specimen should be used for detection within the same day. Otherwise, please store the specimen as follows: Store at 2°C - 8°C for no more than 24 hours; Store at < -20°C for no more than 10 days; Store at < -70°C for long-term, avoiding repeated freeze-thaw cycles.

2. The specimen should be transported using sealed foam box with dry ice.

What's in the box

Components:

  • Detection Buffer 900 μL× 3 tubes (Buffer, dNTPs, Primers,Probes)
  • 400 μL× 1 tube (RNase Inhibitor, UDG,Reverse Transcriptase, Taq DNA polymerase)
  • 250 μL× 1 tube (RNA pseudovirus containing target gene)
  • 250 μL× 1 tube (DEPC-Treated Water)

1. Do not mix the components from different batches for detection.

2. Additional Materials Required: Nucleic acid extraction reagents.

3. Nucleic acid extraction must be performed simultaneously with the Positive control and Negative Control (DEPC-Treated Water) for monitoring the entire procedure to reduce false negative or false positive rates

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