Simple and fast : Extraction can be completed within 10 min
Wide used : Widely suitable for various clinical specimen
Safe : Quickly inactivate virus and release of viral nucleic acid
High yield: High nucleic acid extraction efficiency, making downstream detection more sensitive
• Based on high-effective HiScript II Reverse Transcriptase: with high thermal stability ensuring the complex RNA secondary structure be denatured to obtain longer cDNA.
• A wide range of template input amount of total RNA (from 1 pg to 5 μg). Amplify cDNA fragments as long as 20 kb.
• Anchored Oligo(dT)23VN: further improves the specificity, efficiency and sensitivity of the first-strand cDNA synthesis.
• With different primer selection strategy, the first-strand cDNA products can be widely used for many applications, including cloning, hybridization, PCR amplification, and Real Time PCR
This product provides triplex-detections in a single tube. Specific primers and probes were designed for the detection of conserved region of Virus O gene and N gene, respectively. Internal control (RNAse P gene) provides a nucleic acid extraction procedural control and a secondary negative control. Positive control provides a nucleic acid extraction and a reverse transcription control to validate the entire procedure and reagent