Phanta Max Super-Fidelity DNA Polymerase is a new generation superior enzyme based on Phanta DNA Polymerase for robust PCR with higher fidelity. The unique extension factor, specificity-promoting factors and plateau-inhibiting factor newly added to Phanta Max greatly improve its long-fragment amplification ability, specificity, and PCR yield. Phanta Max is capable of amplifying long fragments such as 40 kb λ DNA, 40 kb plasmid DNA, 20 kb genomic DNA and 10 kb cDNA. The amplification error rate of Phanta Max is 53-fold lower than that of conventional Taq and 6-fold lower than that of Pfu. In addition, Phanta Max has a good resistance to PCR inhibitors and can be used for direct PCR amplifications of bacteria, fungi, plant tissues, animal tissues, and even whole blood samples. Phanta Max contains two monoclonal antibodies inhibiting the 5´→3´ polymerase activity and 3´→5´ exonuclease activity at room temperature, which enable Phanta Max to perform greatly-specific Hot-Start PCRs.
2× Phanta Max Master Mix (Dye Plus) contains Phanta Max Super-Fidelity DNA Polymerase, dNTP, an optimized buffer system, and loading dye. The amplification can start only with the addition of primer and template, thereby easing PCR setup and improving reproducibility. Protective agents in the 2× Phanta Max Master Mix enable the resistance to repeated freeze-thaw cycles. Amplification will generate blunt-ended products, which are compatible with ClonExpress II One Step Cloning Kit Series (Vazyme, Cat. No. #C112, #C113, #C114, #C115).